How to Get a USDOT Number for Your Company To secure a USDOT number, call (888) 414-1874 or apply online at the Registration and Licensing Section of the FMCSA website www.fmcsa.dot.gov. Registration (completion of the MCS-150 Form) is a one-time event that costs $350 and covers all company vehicles. There is a $10,000 mandatory fine for failure to comply with this regulation.